Back to Search
Start Over
Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides.
- Source :
-
Microchimica Acta . Apr2022, Vol. 189 Issue 4, p1-12. 12p. - Publication Year :
- 2022
-
Abstract
- Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 189
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 156500227
- Full Text :
- https://doi.org/10.1007/s00604-022-05272-y