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Peptide-based colorimetric and fluorescent dual-functional probe for sequential detection of copper(Ⅱ) and cyanide ions and its application in real water samples, test strips and living cells.
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . Aug2022, Vol. 276, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- [Display omitted] • A novel dual-functional probe FLH was developed. • FLH has ability to fast (response time <50 s) and sensitively (LOD = 1.5 nM) detect Cu2+. • FLH -Cu2+ complex displayed colorimetric and fluorescent response toward CN−. • FLH was successfully applied to detect Cu2+ and CN− in test strips and real samples. • FLH was almost no toxicity and successfully detected Cu2+ and CN− in living cells. A novel dual-functional peptide probe FLH based on fluorescent "on–off–on" strategy and colorimetric visualization method was designed and synthesized. This new probe exhibited highly selective and rapid detection of Cu2+ with significant fluorescent "turn-off" response, with a visible colorimetric change from yellow to orange. The combination ratio of FLH to Cu2+ (1:1) was determined using ESI-HRMS spectra and Job's plot. The fluorescent emission showed a good linear response (R2 = 0.9986) with a low detection limit of 1.5 nM. In addition, the FLH -Cu2+ complex displayed colorimetric changes and a fluorescent "off–on" response toward CN− over a wide pH range from 7 to 12. This detection behavior was observed within 20 s, with a limit of detection (LOD) for CN− at 12.7 nM. Based on stability and accuracy, FLH was next developed as dual-functional test strips, and was also successfully applied to detect Cu2+ and CN− in two actual water samples. More importantly, the cytotoxicity studies indicated that FLH had good biocompatibility and low toxicity, and was successfully utilized for monitoring Cu2+ and CN− in living cells through fluorescence imaging. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CYANIDES
*WATER sampling
*FLUORESCENT probes
*PEPTIDES
*DETECTION limit
*COPPER
Subjects
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 276
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 156552626
- Full Text :
- https://doi.org/10.1016/j.saa.2022.121222