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Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology.

Authors :
Dolan, Stephen K.
Matilla, Miguel A.
Source :
Microbial Biotechnology. May2022, Vol. 15 Issue 5, p1318-1320. 3p.
Publication Year :
2022

Abstract

Summary: Plasmids are extrachromosomal genetic elements capable of autonomous replication within a host cell. They play a key role in bacterial ecology and evolution, facilitating the mobilization of accessory genes by horizontal gene transfer. Crucially, plasmids also serve as valuable tools in modern molecular biology. Here, we highlight recent articles aimed at implementing standardized plasmid assembly techniques and plasmid repositories to promote open science as well as to improve experimental reproducibility across laboratories. Research focused on assisting these fundamental aims is a further step towards improving standardization in molecular and synthetic biology. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17517907
Volume :
15
Issue :
5
Database :
Academic Search Index
Journal :
Microbial Biotechnology
Publication Type :
Academic Journal
Accession number :
156584033
Full Text :
https://doi.org/10.1111/1751-7915.14012