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Development and Validation of Stability Indicating RP-HPLC Method For the Simultaneous Estimation of Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein in Tablet Dosage Form.
- Source :
-
Indo Global Journal of Pharmaceutical Sciences . 2021, Vol. 11 Issue 2, p85-91. 7p. - Publication Year :
- 2021
-
Abstract
- Purpose: The objective of the proposed method is to develop an economical, accurate, precise, sensitive, reproducible, selective and validated RP-HPLC for simultaneous estimation of Glucosamine sulphate (Glu), Methyl Sulfonyl Methane (MSM) and Diacerein (Dia) in combined dosage form. Method: Mobile phase containing water and acetonitrile in the ratio of 50:50v/v was pumped through the Zodiasil C18 150×4.6mm, 3.5µ column. The flow rate was 0.8ml/min and temperature was maintained at 30°C. The eluents were observed at a wavelength of 210nm using PDA detector. Results: The retention time of Glu, MSM, and Dia were found to be 2.323, 2.842 and 3.453 respectively. The percentage RSD of intraday precision were found to be 0.5 for Glu, 0.2 for MSM and 0.7 for Dia. The percentage RSD of interday precision was found to be 0.7 for Glu, 0.8 for MSM, and 0.9 for Dia. Percentage recovery was found to be 100.30% for Glu, 99.65% for MSM and 100.13% for and Dia. Conclusion: The developed method was simple, precise, accurate, linear, rapid, economical and degradation studies revealed that the method has the ability to separate degradation products in pharmaceutical dosage forms so it can be adopted in regular quality control test for the simultaneous estimation of Glu, MSM, and Dia in tablet dosage form. © 2020 iGlobal Research and Publishing Foundation. All rights reserved. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22491023
- Volume :
- 11
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Indo Global Journal of Pharmaceutical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 156602130
- Full Text :
- https://doi.org/10.35652/IGJPS.2021.112002