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Development of a rapid neutralization assay for the detection of neutralizing antibodies against coxsackievirus B1.
- Source :
-
Diagnostic Microbiology & Infectious Disease . Jun2022, Vol. 103 Issue 2, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- • A rapid Elispot-based neutralization assay against Coxsackievirus B1 (CVB1) was successfully developed. • The CVB1 Nt-Elispot is objective, highly sensitive, efficient, and high-throughput. • The CVB1 Nt-Elispot could be applied in the screening of anti-CVB1 neutralizing monoclonal antibodies. • The CVB1 Nt-Elispot could be used to detect NAb levels against CVB1 in large cohorts. Coxsackievirus B1 (CVB1) is a major pathogen that causes viral myocarditis and aseptic meningitis and is implicated as a cause of type 1 diabetes mellitus. The rapid detection of neutralizing antibodies can help in the prevention and diagnosis of viral infection. The traditional cytopathic effect (CPE)-based neutralization assay (Nt-CPE) is time-consuming and labor-intensive. In this study, an efficient neutralization test based on an enzyme-linked immunospot assay and a monoclonal antibody 2E6 against CVB1 (Nt-Elispot) was developed. In this optimal Nt-Elispot, a multiplicity of infection (MOI) of 1 per well was set as the infection dose, and an incubation time of 18 hours was selected as the checkpoint. Compared with Nt-CPE, Nt-Elispot significantly shortened the detection period and displayed a good correlation with it. This established CVB1 Nt-Elispot could be applied to efficiently screen neutralizing antibodies and evaluate the level of NAb against CVB1 in large cohorts. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07328893
- Volume :
- 103
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Diagnostic Microbiology & Infectious Disease
- Publication Type :
- Academic Journal
- Accession number :
- 156650032
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2022.115676