Performance and selectivity of amphiphilic pillar[5]arene as stationary phase for capillary gas chromatography.

• Amphiphilic pillar[5]arene (P5A-C10-2NH 2) as GC stationary phase. • High resolving performance towards a wide range of analytes and isomers • Multiple recognition interactions with analytes • Quantum chemistry calculation was utilized to assess the separation mechanism. Pillar[ n ]arenes possess highly symmetrical and rigid pillar-shaped architecture with π -electron rich cavity that afford their reliable host-guest recognition interactions towards matched guests. In this work, a novel amphiphilic pillar[5]arene (P5A-C10-2NH 2) was designed, synthesized and employed as the stationary phase for capillary gas chromatography. To date, they have not been reported in the field of chromatography. The P5A-C10-2NH 2 capillary column (10 m × 0.25 mm i.d.) was prepared by static coating method. Its capillary column exhibited moderate polarity and column efficiency of 2265 plates/m determined by naphthalene at 120 °C. As evidenced, the P5A-C10-2NH 2 column achieved advantageous separation performance for a mixture of 24 analytes of diverse types and exhibited different chromatographic selectivity from two pillar[5]arene derivatives columns and commercial HP-35 column with 35%-phenyl-methylpolysiloxane. Moreover, the P5A-C10-2NH 2 column baseline resolved more than a dozen positional and cis - trans isomers. Furthermore, the separation mechanism of P5A-C10-2NH 2 column was discussed by quantum chemical calculations. In addition, the P5A-C10-2NH 2 column had high thermal stability and excellent separation repeatability 0.01–0.04% for run-to-run, 0.03–0.17% for day-to-day and 3.2–3.9% for column-to-column. The special amphiphilic structure and high resolution for various analytes reveal the good potential of pillararenes as a new class of stationary phases for chromatographic analyses. Moreover, the TPG column achieved improved thermal stability over the GIL column and excellent repeatability. [ABSTRACT FROM AUTHOR]