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Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B.
- Source :
-
Analytical Biochemistry . Jul2022, Vol. 648, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- In this work, a lateral flow immunoassay (LFIA) with peptide functionalized gold nanoparticles (termed as biotin- p peptide-AuNPs) has been developed for rapid, semi-quantitative detection of PTP1B activity without using any sophisticated equipment. In this method, the anti-phosphotyrosine (anti-pY) monoclonal antibody and streptavidin were used as test line and control line, respectively. The biotin- p peptide-AuNPs contain 10% biotinylated peptide ligand carry a motif SDGHEpYIYVDP with pY (phosphotyrosine) and 90% pentapeptide (CALNN) ligand, which are used as PTP1B substrates and LFIA labelling probes. The experimental results demonstrate that the as-proposed LFIA with biotin- p peptide-AuNPs exhibits a wide linear range (from 50 ng/mL to 10 μg/mL), a relatively low limit of detection (LOD, 44 ng/mL), and good specificity. In addition, the LFIA with biotin- p peptide-AuNPs has been successfully used to evaluate activity levels of PTP1B in four cell lysates and the detection results exhibit a consistent trend with that of commercial kit. A lateral flow immunoassay with peptide functionalized gold nanoparticles was developed for rapid, semi-quantitative detection of PTP1B activity. [Display omitted] • We developed a LFIA with peptide functionalized AuNPs for rapid, semi-quantitative detection of PTP1B activity. • The LFIA has been successfully used to evaluate activity levels of PTP1B in biological samples (e.g., cell lysates). • The result is in accordance with that of commercial kit. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 648
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 156673389
- Full Text :
- https://doi.org/10.1016/j.ab.2022.114671