Sulfite-based pretreatment promotes volatile fatty acids production from microalgae: Performance, mechanism, and implication.

[Display omitted] • Sulfite-based pretreatment remarkably promoted VFAs production from microalgae. • Acetate proportion of 54.5% was obtained at 200 mg S/L with time of day 8. • More and relatively easy biodegradable organics were released into liquid phase. • Sulfite-induced sulfate-reducing bacteria facilitated the acetate generation pathway. • β-glucanase, β-glucosidase, and acetate kinase activities were accelerated. Volatile fatty acids (VFAs) production from anaerobic fermentation of microalgae is generally constrained by low organics solubilization and poor substrate-availability. In this study, sulfite-based pretreatment was developed to overcome such situation. Experimental results showed that the maximum concentration of VFAs (467.5 mg COD/g VSS) and corresponding acetate proportion (54.5%) was obtained at 200 mg sulfite-S/L with fermentation time of day 8, which was respectively 2.1- and 1.9-fold of control. It was found that after sulfite pretreatment, more and relatively easy biodegradable organics were released into liquid phase, providing available substrate for acid-producing bacteria. The rigid cell wall of microalgae was destroyed, evidenced by the decreased particle size and increased surface area, which made the microalgae more accessible for subsequent hydrolysis and acidification. Meanwhile, the sulfite-induced sulfate-reducing bacteria facilitated the acetate generation pathway. The accelerated activities of β-glucanase, β-glucosidase, and acetate kinase involved in anaerobic fermentation further validated the above results. [ABSTRACT FROM AUTHOR]