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A sensitive electrochemiluminescence aptasensor for Pb2+ detection in soil based on dual signal amplification strategy of aggregation-induced emission and resonance energy transfer.
- Source :
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Electrochimica Acta . Jul2022, Vol. 421, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
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Abstract
- • An on-off-on ECL aptasensor was developed based on dual signal amplification strategy. • Aggregation-induced emission behavior of AuNCs provided the first signal-on state. • The signal-off state was realized by resonance energy transfer between RhB and AuNCs. • The Pb2+-induced second "signal-on" state was obtained by blocking RET process. • The proposed aptasensor showed excellent analytical performance for Pb2+ analysis. In the present work, based on dual signal amplification strategy of aggregation-induced emission (AIE) and resonance energy transfer (RET), a highly sensitive and selective "on-off-on" output model electrochemiluminescence (ECL) aptasensor for Pb2+ detection was constructed. In this approach, the first "signal-on" with a high and stable ECL signal was obtained by gold nanoclusters (AuNCs)-modified glassy carbon electrode surface with triethylamine as co-reactant. The AIE behaviour of AuNCs amplified ECL emission compared with AuNCs in solution. Later, the "signal-off" was activated by rhodamine B (RhB) quenched the AuNCs signal due to good spectral overlap between ultraviolet-visible absorption peak of RhB and ECL spectrum of AuNCs. The combination of dual signal amplification strategy provided a high signal to background ratio, leading to the improved sensitivity of the aptasensor. In the presence of Pb2+, its specific binding to the aptamer induced the departure of RhB from the sensing interface, which resulted in the favourable recovery of ECL signal (Second "signal-off"). Therefore, the proposed ECL aptasensor exhibited a wide detection linear range from 1.0 × 10−10 to 1.0 × 10−4 M. Prospectively, the development dual signal amplification strategy could provide a novel sensing strategy for Pb2+ detection. [Display omitted] [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00134686
- Volume :
- 421
- Database :
- Academic Search Index
- Journal :
- Electrochimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 157000993
- Full Text :
- https://doi.org/10.1016/j.electacta.2022.140463