Fabrication and research of Mg(OH)2/PCL/PVP nanofiber membranes loaded by antibacterial and biosafe Mg(OH)2 nanoparticles.

In order to fabricate antibacterial nanofiber membranes for tissue engineering field, the oxidative damage of magnesium hydroxide nanoparticles (Mg(OH) 2 NPs) against the model system of Escherichia coli (E. coli) was researched by measuring the oxidative stress response, oxidative stress products and glucose metabolism. The toxicity of Mg(OH) 2 NPs to a normal biological system was evaluated in vivo through the acute oral toxicity, blood physiological and serum biochemical markers and acute skin irritation, indicating that the Mg(OH) 2 NPs exhibited great biosafety. The Mg(OH) 2 NPs with antibacterial property and biosafety were loaded on PCL/PVP nanofiber substrates through electrospinning technology to fabricate the Mg(OH) 2 /PCL/PVP nanofiber membranes. The results of characterization and antibacterial test showed that the Mg(OH) 2 /PCL/PVP nanofiber membranes with 8.0 wt% Mg(OH) 2 NPs present excellent antibacterial rate of 100% against E. coli that the Mg(OH) 2 NPs can be physically and evenly loaded on nanofiber substrates. • Oxidative damage of Mg(OH) 2 NPs against E. coli was evaluated. • Biosafety of Mg(OH) 2 NPs was evaluated in vivo. • Mg(OH) 2 /PCL/PVP nanofiber membranes were fabricated for the first time. • Mg(OH) 2 NPs were loaded on nanofiber substrates by electrospinning method. [ABSTRACT FROM AUTHOR]