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A c-MWCNTs/AuNPs-based electrochemical cytosensor to evaluate the anticancer activity of pinoresinol from Cinnamomum camphora against HeLa cells.
- Source :
-
Bioelectrochemistry . Aug2022, Vol. 146, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- • Cervical carcinoma is a leading cause of mortality in women worldwide. • Lignans, as phytochemicals, have therapeutic potential for cancer cell lines. • An electrochemical cytosensor evaluated pinoresinol activity on HeLa cells. • Pinoresinol induced apoptosis in HeLa cells and hindered HeLa cell cycle. • MTS, flow cytometry, and fluorescent imaging confirmed the cytosensor results. A sensitive, simple, label-free electrochemical cytosensor was developed to evaluate the anticancer activity of pinoresinol against human cervical carcinoma (HeLa) cells. HeLa cells were immobilised on carboxylated multi-walled carbon nanotubes (c-MWCNTs)/gold nanoparticles (AuNPs) nanocomposite-modified glassy carbon electrodes. Scanning electron microscopy, transmission electron microscopy, Fourier transform-infrared spectroscopy, and X-ray diffractometry were used to characterise the morphology, crystallinity, and composition of the nanocomposites. Cyclic voltammetry was used to characterise and optimise the cytosensor. Both c-MWCNTs and AuNPs increased the electron-transfer rate between the HeLa cells and the electrode, retaining good cell compatibility. The concentration of HeLa cells immobilised on the nanocomposite electrode exhibited a good correlation with the impedance values determined by electrochemical impedance spectroscopy in the range of 102–106 cells/mL (detection limit of 102 cells/mL) with R2 = 0.975. HeLa cells were then immobilised on the nanocomposite electrode at a concentration of 104 cells/mL, and it was used as a cytosensor. The anticancer activity of pinoresinol from Cinnamomum camphora was evaluated by determining the median inhibitory concentration using the proposed cytosensor. For comparison, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and fluorescent imaging were simultaneously performed, which confirmed the results of the cytosensor. The cytosensor is promising for future applications in anticancer drug screening. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15675394
- Volume :
- 146
- Database :
- Academic Search Index
- Journal :
- Bioelectrochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 157456008
- Full Text :
- https://doi.org/10.1016/j.bioelechem.2022.108133