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A rhodol-derived probe for intracellular biothiols imaging and rapid labelling of sulfhydryl-containing proteins.
- Source :
-
Sensors & Actuators B: Chemical . Sep2022, Vol. 367, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- Biological thiols are crucial in biological processes. Therefore, a probe capable of targeting and imaging biothiols in living cells, as well as rapidly labelling the thiols in proteins, is desirable and imperative. Herein, we designed and synthesized FRB-T containing α , β -unsaturated ketones to recognize thiols for detecting biothiols and selectively labelling the sulfhydryl-containing proteins. The probe initially exhibited weak fluorescence and then performed 1, 4-Michael addition reaction between its α , β -unsaturated ketone and thiols, along with the spiro ring-opening, leading to remarkable fluorescence enhancement and colour changes. The specificity of FRB-T towards thiols was high, and its detection limits were 70 nM for cysteine (Cys) and 1.51 μg/mL for ovalbumin (OVA). Furthermore, FRB-T has also been successfully applied to biothiols imaging in Hela cells, and rapid labelling of the sulfhydryl-containing proteins without washing step. [Display omitted] • A biothiol-specific probe with strong fluorescence enhancement and colorimetric response. • The probe owns low LOD of 70 nM and 1.51 μg/mL for Cys and OVA respectively. • A new type of reagent for labelling sulfhydryl-containing proteins without washing. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 367
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 157499413
- Full Text :
- https://doi.org/10.1016/j.snb.2022.132148