Investigation of the effect of antibiotics on 5-formylcytosine content in mazie seedling tissues based on photoelectrochemical biosensor.

Given the improved photoactivity of Bi 2 S 3 by MXene, an article photoelectrochemical biosensor was designed for 5-formyl-2′-deoxycytidine (5fdCTP) detection, where Bi 2 S 3 : MXene was selected as photoactive material, polydopamine was used as solid electron donor and 5fdCTP capture reagent, calcined ZIF-8(C-ZIF-8) was chosen as the artificial enzyme. With the catalyzed by C-ZIF-8, 4-chloro-1-naphthol was allegro oxidized by H 2 O 2 to form the insoluble benzo-4-chlorohexadienone, and then deposited on the surface of the electrode, Resulting in a decrease of the PEC response. Under the optimum conditions, the sensor has a linear range of 0.001–200 nM and a detection limit of 0.51 pM (3σ). The suitability of the developed method was appraised by investigating the effect of antibiotics on 5fdCTP content in the genomic DNA of the roots of maize seedlings. As a new detection platform, the application of this approach can be expanded to investigative the impact of other pollutants on the content of 5fdCTP in plant or animal tissues, explore the feasibility of 5fdCTP as a new indicator for the ecotoxicological effect of pollutants, and the photoelectrochemical method as a new assessment technique for the ecotoxicological effects of pollutants. [Display omitted] • The accordion-like MXene greatly improves the photoelectric activity of Bi 2 S 3. • The effect of antibiotics on 5fC expression in maize seedlings was investigated. • 5fC was detected by photoelectrochemical method using Bi 2 S 3 /MXene as photoactive material. • 5fC was recognized by PDA and C-ZIF-8 was used as photoelectrochemical signal inhibitor. [ABSTRACT FROM AUTHOR]