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A Simple Fluorescence Affinity Assay to Decipher Uranyl‐Binding to Native Proteins.
- Source :
-
Angewandte Chemie International Edition . 6/27/2022, Vol. 61 Issue 26, p1-5. 5p. - Publication Year :
- 2022
-
Abstract
- Determining the affinity of proteins for uranyl is key to understand the toxicity of this cation and to further develop decorporation strategies. However, usual techniques to achieve that goal often require specific equipment and expertise. Here, we propose a simple, efficient, fluorescence‐based method to assess the affinity of proteins and peptides for uranyl, at equilibrium and in buffered solution. We first designed and characterized an original uranyl‐binding fluorescent probe. We then built a reference scale for uranyl affinity in solution, relying on signal quenching of our fluorescent probe in presence of high‐affinity uranyl‐binding peptides. We finally validated our approach by re‐evaluating the uranyl‐binding affinity of four native proteins. We envision that this tool will facilitate the reliable and reproducible assessment of affinities of peptides and proteins for uranyl. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENT probes
*PROTEINS
*FLUORESCENCE
*PEPTIDES
*BIOINORGANIC chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 61
- Issue :
- 26
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 157549321
- Full Text :
- https://doi.org/10.1002/anie.202203198