Back to Search Start Over

Expression profile analysis in cells overexpressing DRPLA cDNA to explore the roles of DRPLAp as a transcriptional coregulator.

Expression profile analysis in cells overexpressing DRPLA cDNA to explore the roles of DRPLAp as a transcriptional coregulator.

Authors :
Hatano, Keiko
Date, Hidetoshi
Ishiura, Hiroyuki
Matsukawa, Takashi
Tanaka, Masaki
Mitsui, Jun
Goto, Jun
Yoshimura, Jun
Doi, Koichiro
Morishita, Shinichi
Tsuji, Shoji
Source :
Neurology & Clinical Neuroscience. Jul2022, Vol. 10 Issue 4, p210-217. 8p.
Publication Year :
2022

Abstract

Background: Dentatorubral‐pallidoluysian atrophy (DRPLA) is an autosomal dominant neurodegenerative disorder caused by the unstable expansion of CAG repeats encoding polyglutamine stretches in DRPLA gene (ATN1). DRPLAp, the gene product of ATN1, is located in the nucleus and has been shown to function as a transcriptional coregulator. Its target genes, however, remain largely unknown. Aim: To elucidate the physiological functions of DRPLAp based on determination of the target genes regulated by wild‐type DRPLAp. Methods: Using the cultured cells expressing wild‐type full‐length DRPLA cDNA (Q19) encoding DRPLAp under the control of the doxycycline (Dox)‐On system, we searched for genes whose expression levels were regulated by wild‐type DRPLAp (Q19). Results: On the basis of detailed RNA‐seq analyses, we identified seven up‐regulated genes and five down‐regulated genes in Q19 cells. In gene ontology analysis, the up‐regulated differentially expressed genes formed clusters featuring skeletal system morphogenesis, transcriptional regulation, and RNA metabolism, which is consistent with the recent report that the mutations in ATN1 lead to recognizable facial form and variable congenital anomalies. Conclusion: These findings support that DRPLAp may play a role as a transcriptional coregulator involved in the regulation of development. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20494173
Volume :
10
Issue :
4
Database :
Academic Search Index
Journal :
Neurology & Clinical Neuroscience
Publication Type :
Academic Journal
Accession number :
157892290
Full Text :
https://doi.org/10.1111/ncn3.12607