Back to Search
Start Over
Apigenin sensitizes radiotherapy of mouse subcutaneous glioma through attenuations of cell stemness and DNA damage repair by inhibiting NF-κB/HIF-1α-mediated glycolysis.
- Source :
-
Journal of Nutritional Biochemistry . Sep2022, Vol. 107, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- We used radioresistant SU3-5R stem cells-inoculated subcutaneous glioma model to investigate the radiosensitization effect of apigenin. After treatment of glioma with apigenin 20 mg/kg for 12 days, irradiation 8 Gray twice or their combination, the tumor volume and weight were decreased, especially in the combination group. Apigenin inhibited the activities of glycolytic enzymes and expressions of nuclear factor kappa B (NF-κB) p65, hypoxia inducible factor-lα (HIF-1α), glucose transporter (GLUT)-1/3 and pyruvate kinase isozyme type M2 (PKM2) proteins in tumor tissues. After treatment of SU3-5R cells with apigenin 7.5 µM, the fluorescence intensity of CD133 positive cells was decreased, the percentage of cells with comet tails was increased, and the expressions of lipopolysaccharide-induced NF-κB p65, HIF-1α, GLUT-3 and PKM2 proteins were reduced. These results demonstrate that apigenin can sensitize the radiotherapy of glioma via the attenuations of cell stemness and DNA damage repair by inhibiting NF-κB/HIF-1α-mediated glycolytic enzymes and protein expressions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09552863
- Volume :
- 107
- Database :
- Academic Search Index
- Journal :
- Journal of Nutritional Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 158157981
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2022.109038