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A near-infrared ratiometric fluorescent probe with large stokes shift for rapid detection of ClO− in living cells.
- Source :
-
Journal of Molecular Structure . Nov2022, Vol. 1267, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- • A near infrared AIE probe for visualization of ClO−and LDs at the cellular level. • Specifically recognize ClO− with high sensitivity, large Stokes shift. • The reaction between the T and ClO− was accelerated by loading the probe with protein. Both of lipid droplets (LDs) and hypochlorous acid/hypochlorite (HClO/ClO−) play important roles in regulating cell metabolism and maintaining normal operation of life activities. Here, we report the synthesis of probe T, which is a typical compound with AIE properties. The probe T can not only detect ClO− by proportional fluorescence, but also specifically target LDs. T displayed an excellent spectral performance with a long emission wavelength (715 nm), large stokes shift (211 nm), wide pH response range. When it encountered ClO−, the fluorescence color changed from red to blue with a large fluorescence signal shift (from 715 nm to 430 nm) and good sensitivity (detection limit: 0.102 μM). More interestingly, we successfully advanced the response speed of T to ClO− by loading the AIE probe with protein. Additionally, cell imaging experiments demonstrated that T was perfectly utilized to visualize exogenous/endogenous ClO− in cells and possessed an excellent LDs-targeting ability. [Display omitted] [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENT probes
*STOKES shift
*CELL imaging
*CELL metabolism
*DETECTION limit
Subjects
Details
- Language :
- English
- ISSN :
- 00222860
- Volume :
- 1267
- Database :
- Academic Search Index
- Journal :
- Journal of Molecular Structure
- Publication Type :
- Academic Journal
- Accession number :
- 158208286
- Full Text :
- https://doi.org/10.1016/j.molstruc.2022.133570