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The phenylcoumaran benzylic ether reductase gene PtPCBER improves the salt tolerance of transgenic poplar through lignan-mediated reactive oxygen species scavenging.

Authors :
Wei, Mingxing
Yu, Chunyan
Ge, Bingkun
Liu, Yue
Zhang, Haiyang
Duan, Chunli
Zhang, Juan
Mao, Tingting
Huang, Huiqing
Xie, Yinshuai
Gao, Hongsheng
Zhang, Hongxia
Wang, Aike
Yi, Yanjun
Source :
Environmental & Experimental Botany. Sep2022, Vol. 201, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

Phenylcoumaran benzylic ether reductase (PCBER) in the phenylpropane metabolic pathway plays a crucial role in controlling plant growth and development. However, its biological function in response to abiotic stress in perennial trees is still largely obscure. In this work, a phenylcoumaran benzylic ether reductase gene, PtPCBER , isolated from poplar was overexpressed in poplar. The growth and salt resistance of transgenic plants were investigated, and the possible regulatory mechanism of PtPCBER in response to abiotic stresses was verified. PtPCBER was constitutively expressed in various tissues and organs with a predominant expression in xylems. Overexpression of PtPCBER augmented the salt and oxidative stress tolerance of transgenic poplar plants. The increased salt tolerance was associated with a relatively lower chlorphyll loss and Na+ accumulation, and a higher antioxidant enzyme activity and stress gene expression, in the leaves of transgenic plants. Further metabolomic analyses revealed that overexpression of PtPCBER cut down the accumulation of coniferyl alcohols and lignans which function in the reactive oxygen species (ROS) scavenging pathway to alleviate the damage caused by oxidative stress in the leaves of transgenic plants. Taken together, our results suggest that PtPCBER has an important function in plant response to salt stress, and it could be used as an ideal candidate gene for the genetic engineering of woody plants with enhance resistance to multiple abiotic stresses through improving the scavenging capacity of ROS. • Overexpression of PtPCBER promoted salt tolerance in transgenic poplar. • The salt tolerance of transgenic plants was associated with ion homeostasis changes. • The reactive oxygen species scavenging capacity was increased in transgenic poplar. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00988472
Volume :
201
Database :
Academic Search Index
Journal :
Environmental & Experimental Botany
Publication Type :
Academic Journal
Accession number :
158262876
Full Text :
https://doi.org/10.1016/j.envexpbot.2022.104946