Reporting of mycetoma cases from skin and soft tissue biopsies over a period of ten years: A single center report and literature review from Pakistan.

Background: Mycetoma is an important neglected tropical disease associated with debilitation, disfigurement and death if not diagnosed and treated adequately. In Pakistan, mycetoma cases have frequently been diagnosed in histopathology and microbiology laboratories. However, there is scarcity of published data from this country. Therefore, the objectives of this study were to evaluate the frequency and type of mycetoma reported in skin and soft tissue biopsies from a single center over 10 years and review of published literature from Pakistan. Method: This descriptive observational retrospective study was conducted at the Aga Khan University Hospital laboratory, Karachi, Pakistan. Laboratory data from 2009–2018 of skin and soft tissue biopsies with positive findings of mycetoma were retrieved from hospital information system. The variables for statistical analysis were age and gender of patient, anatomical site of lesion, residence of patient (geographical location) in the country, etiologic agents of mycetoma and significant gross and microscopic histopathological findings. The data was entered, and descriptive epidemiologic assessment was carried out using MS excel 2013. Geographical information system was used for mapping the location. Literature review of mycetoma cases reported from Pakistan was done on PubMed, Google search and PakMediNet from 1980 till April 2019. Result: During ten years of study period, 89 skin and soft tissue biopsies were reported as mycetoma, majority were eumycetoma [n = 66/89 (74%)] followed by actinomycetoma [n = 23/89 (26%)]. Involvement of lower limb was predominantly observed [n = 74/89 (83%)] in which foot had significant contribution [n = 65/74 (88%)]. Only 18 specimens were submitted for microbiological assessment and six grew agents of mycetoma, with Madurella mycetomatis reported in only three. Well-formed granuloma formation was observed in only 26%[n = 23/89] of cases. Specific geographical location was not identified, and cases were reported from across the country. From Pakistan, only two original papers and 7 case reports were available in published literature. Conclusion: This single center study reports a handful of cases of mycetoma from Pakistan. We conclude that the index of suspicion should remain high among treating surgeons and physicians and clinical laboratories should improve their diagnostic capacity and skills. This will have a great impact on disease outcome and patient's life. Author summary: World Health Organization (WHO) has included Mycetoma in the neglected tropical diseases. Epidemiological data of mycetoma cases from Pakistan is not available. Although physicians and surgeons frequently see cases of mycetoma in their clinical practice, but poor socioeconomic conditions of patients restrict laboratory diagnosis such as histopathological and microbiological confirmation of disease. This leads to disability, disfigurement and at times limb loss or mortality. Through this study we are documenting the diagnosed cases of mycetoma from specimens submitted to our laboratory. The diagnosis was mainly made by histopathological examination of samples. It is disappointing to note that mycetoma was not included in the differential diagnosis list of clinicians. Probably, that is the reason that very few samples were submitted to the microbiology laboratory for the culture to find out etiologic agents of eumycetoma and actinomycetoma. Overall, this study has highlighted the gaps in the clinical as well as laboratory diagnosis of this disease and case management. [ABSTRACT FROM AUTHOR]