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Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 12/28/2004, Vol. 101 Issue 52, p18012-18017. 6p. - Publication Year :
- 2004
-
Abstract
- Sister chromatids in mammalian cells remain attached mostly at their centromeres at metaphase because of the loss of cohesion along chromosome arms in prophase. Here, we report that Bub1 retains centromeric cohesion in mitosis of human cells. Depletion of Bub1 or Shugoshin (Sgo1) in HeLa cells by RNA interference causes massive missegregation of sister chromatids that originates at centromeres. Surprisingly, loss of chromatid cohesion in Bubi and Sgo1 RNA-interference cells does not appear to require the full activation of separase but, instead, triggers a mitotic arrest that depends on Mad2 and Aurora B. Bub1 maintains the steady-state levels and centromeric localization of Sgo1. Therefore, Bub1 protects centromeric cohesion through Shugoshin in mitosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SISTER chromatid exchange
*CELLS
*CHROMOSOMES
*RNA
*MITOSIS
*RIBOSE
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 101
- Issue :
- 52
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 15828997
- Full Text :
- https://doi.org/10.1073/pnas.0408600102