热碱法提取制革污泥蛋白质及表征.

The protein hydrolysates were prepared by the thermal-alkaline treatment of tannery sludges, and then the proteins of tannery sludge were successfully extracted by the acidification method. The results showed that the extraction rate of sludge proteins increased and then decreased with the increase of NaOH dosage, time and temperature. The optimum temperature, time and NaOH dosage for extracting sludge proteins were 150 °C, 60 min and 9 g/L respectively, resulting in the highest extraction rate of 71. 2%. The interaction o£ temperature, temperature and time is the most important factor to affect the extraction rate o£ sludge proteins. The purity, total chromium concentration, common amino acid content of extracted tannery sludge proteins is 83. 01%, 1.5 mg/kg and 31. 09%, respectively. In addition, the sludge proteins have a low content of inorganic salts and ashes. The molecular weight of tannery sludge proteins ranges from 5 to 35 kDa. [ABSTRACT FROM AUTHOR]