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Administration time-dependent effects of poly (I:C) on antioxidant and immune responses along the diurnal time scale in zebrafish.
- Source :
-
Chronobiology International: The Journal of Biological & Medical Rhythm Research . Sep2022, Vol. 39 Issue 9, p1256-1267. 12p. - Publication Year :
- 2022
-
Abstract
- The circadian clock of vertebrates regulates many biological processes, including the immune system. This paper investigated whether responsiveness to poly (I:C), a synthetic analog of double-stranded RNA used as an immunostimulant, exhibits day/night differences in zebrafish. Fish were intraperitoneally (IP) injected with either phosphate-buffered saline (PBS) or poly (I:C) at two different time points: "Zeitgeber Time" (ZT) 4 (day) and ZT16 (night). Then, 6 h later, fish were euthanized, and tissue samples (skin, liver and kidney) were collected. A control group (intact fish) was also sampled at the same time points. The effect of poly (I:C) on the expression of antioxidant and immune genes was time-of-day-dependent, and the response was stronger following poly (I:C) administration in the day than at night. Time-dependent differences were observed for some genes in the PBS and control groups. However, these differences were tissue-specific. In liver, almost all the genes were affected by time of day. In kidney, poly (I:C) affected the expression of all the gene markers regardless of administration time. These findings highlight the importance of considering the time to administer poly (I:C) when evaluating the fish immune response. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IMMUNE response in fishes
*IMMUNE response
*BRACHYDANIO
*DOUBLE-stranded RNA
Subjects
Details
- Language :
- English
- ISSN :
- 07420528
- Volume :
- 39
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Chronobiology International: The Journal of Biological & Medical Rhythm Research
- Publication Type :
- Academic Journal
- Accession number :
- 158361225
- Full Text :
- https://doi.org/10.1080/07420528.2022.2093735