The anticardiac fibrosis of total alkaloids of Plumula nelumbinis by regulating circulating lipidomic profile: In vivo study.

Plumula nelumbinis has great medicinal potential as a herbal tea and traditional drug in China. This study was aimed to evaluate the anticardiac fibrosis of the total alkaloids of P. nelumbinis (TAP). TAP at 50 mg/kg/day significantly ameliorated isoproterenol‐induced cardiac fibrosis in mice (p <.05). The circulating lipidomics study revealed that TAP improved the lipid metabolism dysfunction in cardiac fibrosis. Meanwhile, TAP suppressed the lipid accumulation, decreased MDA level (p <.01) in heart, and increased FFA level (p <.01). Furthermore, integrating lipidomics, chemical profiles and pharmacology network analysis found that AMPK and PI3K/Akt signaling pathways were the potential targeted pathway by TAP to regulate lipid metabolism dysfunction including glycerophospholipid metabolism. Above all, TAP provided a potential anticardiac fibrosis effect partly through regulation of lipid profiles. Practical applications: The total alkaloids of Plumula nelumbinis (TAP) suppressed ISO‐induced cardiac fibrosis in mice.Network pharmacology analysis and experiments revealed that TAP‐regulated AMPK and PI3K/Akt signaling pathway to improve lipid metabolism disorder in cardiac fibrosis.This study provides evidence to the therapeutic potential of TAP in the treatment of ISO‐induced cardiac fibrosis and could be a drug candidate for prevention and treatment of cardiac fibrosis. [ABSTRACT FROM AUTHOR]