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Pharmacokinetics-derived absorbed components responsible for Guizhi-Fuling capsule target PI3K/Akt-Erk to exert an anti-dysmenorrhea effect.
- Source :
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Journal of Ethnopharmacology . Oct2022, Vol. 297, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
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Abstract
- Guizhi-Fuling capsule (GZFL), a well-known herbal remedy, has been widely used to treat primary dysmenorrhea (PD). Hence, systematic identifying multiple active ingredients and the involved mechanism is essential and urgently needed for GZFL. Aim of the study : This study was planned to assess the pharmacokinetics of GZFL in rats, and identify whether these GZFL-derived absorbed components (ACs) contribute to the efficacy of source herbs and relevant mechanism. The in vivo pharmacokinetic profile of 11 phytochemicals and 13 metabolites in healthy and PD rats were evaluated using liquid chromatography with mass spectrometry (LC-MS/MS). Whereafter, the introduced contribution strategy assessed ACs' effect (doses = their contents in GZFL) in PD rats with the mechanism. The pharmacokinetic profiles of prototypes and metabolites differed in healthy and PD rats. As a main proxy of GZFL, 11ACs exerted an anti-PD effect (improvement of indexes for writhing latency, writhing time, PGF 2α /PGE 2 , TXB 2 /6-keto-PGF 1α and β-EP) by regulating PI3K-Akt/ERK pathway. As a paradigmatic example, 11ACs contributed an average of 113.55% to GZFL in terms of anti-PD efficacy, providing an approach to rapidly, accurately and consistently identify the bioactive components and their pathway from herbs. [Display omitted] • Pharmacokinetics of phytochemicals and metabolites was explored in healthy/PD rats. • 11compounds (doses = content in herbs) exerted average ∼113.55% anti-PD effects. • PI3K-AkT/ERK participated in ACs-showing anti-PD efficacy, validated by agonists. • The dose and contribution strategy can become paradigmatic for multi-herbs study. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOLOGICAL models
*HERBAL medicine
*IN vivo studies
*ANIMAL experimentation
*LIQUID chromatography
*TREATMENT effectiveness
*RATS
*PHYTOCHEMICALS
*CELLULAR signal transduction
*DYSMENORRHEA
*MASS spectrometry
*DESCRIPTIVE statistics
*CHINESE medicine
*METABOLITES
*PHARMACOKINETICS
*THERAPEUTICS
*EVALUATION
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 297
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 158423337
- Full Text :
- https://doi.org/10.1016/j.jep.2022.115525