Nano-elektrokimyasal Biyosensörler Kullanılarak DNA ile Doksorubisin Etkileşiminin Araştırılması.

In this study, the interaction between double-stranded deoxyribonucleic acid (dsDNA) and doxorubicin (DOX) was investigated using nano-electrochemical biosensors by differential pulse voltammetry. The biosensor surface was prepared from a solution containing cetyl trimethylammonium (cab) and carbon nanotubes (cnt) with the synthesis of poly(sab)-knt by electrodeposition method. Voltammetric signals of dsDNA electroactive bases were used as indicators to investigate DNA-drug interactions. It was observed that the oxidation peak currents of both guanine and adenine bases decreased after drug-DNA interaction. The linear range for DOX was found to be between 0.39-25 µg mL-1 and limit of detection was found as 0.26 µg mL-1. Besides, the drug-DNA interaction was demonstrated spectrochemically by UV-vis spectroscopy. Electrochemical and spectrochemical studies have shown that the interaction mechanism between DOX and dsDNA occurs with the intercalation mode. [ABSTRACT FROM AUTHOR]