Fabrication and characteristic studies of doped metal oxide-silane magnetic nanocomposite for enhancement of stability of α-amylase.

Ubiquitous nanomaterials have been extensively involved in multifarious biotransformation of diverse organic and synthetic techniques. This research study describes the robust immobilization of α-amylase on magnetic nanoparticles of magnesium ferrite (MgFO) functionalized with silane. The XRD patterns, FESEM and HRTEM images were analysed to study structural and morphological features. The crystallite size of MgFO is found to be 12 nm. The FTIR results confirmed the covalent attachment of the enzyme with the MgFO and VSM and Mössbauer spectrometric graphs were evaluated to study the magnetic behaviour of the prepared samples. The Kinetic parameters, reusability, storage capacity and catalytic activity of the enzyme at various reaction time, pH and temperature conditions before and after attachment with MgFO were examined to confirm the successful immobilization process. The reusability of the enzyme on modified MgFO is found to be good (> 50%) even after 12 consecutive usability cycles and also retain 50% catalytic efficiency over 30 days storage period. [ABSTRACT FROM AUTHOR]