Back to Search Start Over

An observational study showing dipeptidyl peptidase-4 (DPP-4) activity and gene expression variation in chronic liver disease (CLD) patients from a tertiary care hospital of Eastern India.

Authors :
Barkondaj, Bikramjit
Nargis, Titli
Chakrabarti, Partha
Mukhopadhyay, Satinath
Biswas, Kalidas
Ganguly, Dipyaman
Chaterjee, Chandan
Sengupta, Nilanjan
Hazra, Avijit
Source :
Indian Journal of Endocrinology & Metabolism. May/Jun2022, Vol. 26 Issue 3, p245-251. 7p.
Publication Year :
2022

Abstract

Introduction: The studies in animal models of cirrhosis suggest that dipeptidyl peptidase type 4 (DPP-4) enzymes play a crucial role in disease pathogenesis. In this clinical observational study, activity of DPP-4 and related gene expression were analysed in chronic liver disease patients. Objectives: To understand the DPP-4 enzyme activity variation in the common types of chronic liver disease by assessing plasma and peripheral blood mononuclear cell (PBMC) DPP-4 activity and comparing with healthy controls and to explore DPP-4 gene expression in PBMC. Methods: We recruited 130 study subjects in four cohorts—46 nonalcoholic fatty liver disease (NAFLD), 23 non-alcoholic cirrhosis (NAC) excluding viral aetiology, 21 alcoholic liver disease (ALC), and 40 control subjects. Blood samples were analysed for relevant biochemical parameters and plasma DPP-4 activity. PBMC fraction was used for the DPP-4 activity assay and gene expression analysis. Results: We found that lower plasma DPP-4 activity among patient cohorts but this was not statistically significant. The PBMC DPP-4 activity was significantly lower in NAFLD cohort. In the same cohort, DPP-4 gene expression in PBMC fraction was significantly increased (P < 0.05). There was significant correlation between plasma DPP-4 activity and liver injury marker alanine aminotransferase (ALT) among NAFLD (rho = 0.459, P < 0.01), NAC (rho = 0.475, P < 0.05), and ALC (rho = –0.572, P < 0.01) patients. Plasma DPP-4 activity modestly predicted ALT plasma level (beta coefficient = 0.489, P < 0.01). Conclusions: The PBMC DPP-4 activity and DPP-4 gene expression gets significantly altered in NAFLD patients. Plasma DPP-4 activity also shows correlation with ALT levels in CLD patients. The role of DPP-4 in disease pathology in NAFLD and other forms of CLD needs to be explored. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22308210
Volume :
26
Issue :
3
Database :
Academic Search Index
Journal :
Indian Journal of Endocrinology & Metabolism
Publication Type :
Academic Journal
Accession number :
158817946
Full Text :
https://doi.org/10.4103/ijem.ijem_139_22