A highly selective colorimetric and fluorescent probe Eu(tdl)2abp for H2S sensing: Application in live cell imaging and natural water.

A fluorescent probe Eu(tdl) 2 abp for efficient and specific recognition of hydrogen sulfide (H 2 S) was successfully synthesized and characterized by NMR and MS. Eu(tdl) 2 abp showed "on-off" fluorescence signals for H 2 S and its color changes could be identified with naked eyes. Eu(tdl) 2 abp had short response time (2 min) to H 2 S, high selectivity and good anti-interference, large stokes shift (207 nm). In various samples, when H 2 S existed, the azide group was reduced to amine group, resulting in closed-circuit fluorescence signal, and the fluorescence intensity reached the degree of quenching without being affected by other interference. At the same time, there was a good linear relationship between relative fluorescence intensity and H 2 S concentration. By calculation, the high sensitivity (LOD) is 0.64 μM. Eu(tdl) 2 abp was successfully used for the sensitive detection of H 2 S in natural water and living cells. [Display omitted] • A probe Eu(tdl) 2 abp was successfully prepared, synthesized, and applied for H 2 S detection. • Eu(tdl) 2 abp can perform high efficiency and specificity colorimetric and fluorescent double signal recognition for H 2 S. • The Eu(tdl) 2 abp has been successfully used for imaging H 2 S in living cells and natural water. Using 4-([2,2′: 6′, 2′- terpyridin] -4′-yl) -N, N-dimethylaniline (tdl) as auxiliary ligand and 6-azido-2,2′-bipyridine (abp) as recognition ligand, a europium complex fluorescent probe Eu(4-([2,2′: 6′, 2′-terpyridin] -4′ -yl) -N, N-dimethylaniline) 2 –6-azido-2,2′-bipyridine Eu(tdl) 2 abp for efficient and specific recognition of hydrogen sulfide (H 2 S) was successfully synthesized and characterized by NMR and MS. Eu(tdl) 2 abp represented "on-off" fluorescence signals for H 2 S and its color changes could be identified with naked eyes. Eu(tdl) 2 abp had short response time (2 min) to H 2 S, high selectivity and good anti-interference, large stokes shift (207 nm). In various samples, when H 2 S existed, the azide group was reduced to amine group, resulting in closed fluorescence signal, and the fluorescence intensity reached the degree of quenching without being affected by other interference. At the same time, there was a good linear relationship between relative fluorescence intensity and H 2 S concentration with the detection limit (LOD) of 0.64 μM. The sensing mechanism of Eu(tdl) 2 abp to detect H 2 S was characterized by 1H NMR and HR-MS. Eu(tdl) 2 abp was used with success for the sensitive detection of H 2 S in natural water and living cells. [ABSTRACT FROM AUTHOR]