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Transforming growth factor-beta 1 (TGF-β1)-mediated inhibition of glial cell proliferation and down-regulation of intercellular adhesion molecule--1 (ICAM-1) are interrupted by interferon-gamma (IFN-γ).
- Source :
-
Clinical & Experimental Immunology . Mar1996, Vol. 103 Issue 3, p475-481. 7p. - Publication Year :
- 1996
-
Abstract
- We utilized a model of myelin basic protein (MBP) activation in vivo and MBP-stimulated cultures in vitro to study the influence of TGF-β1 on glial cell proliferation and ICAM-l/leucocyte function-associated antigen-1 (LFA-1) expression, and to observe the antagonistic effects of TGF-β1 and IFN-γ. TGF-β1 inhibited MBP-stimulated and MBP-activated glial cell proliferation, especially in MBP-stimulated separated microglia and astrocytes, and down-regulated the expression of ICAM-1 on MBP-stimulated glial cells and .separated microglia. ICAM-1 expression on MBP-activated glial cells was intensely suppressed, whereas its expression on MBP-stimulated astrocytes was not influenced. TGF-β1 had no effect on LFA-1 expression. In contrast, IFN-γ up-regulated ICAM-1 expression, but inhibited proliferative response on MBP-stimulated glial cells when cultured without TGF-Tbeta;1. Examination of TGF-β1 and IFN-γ interactions revealed that TGF-β1-mediated inhibition of proliferation and down-regulation of ICAM-1 on glial cells were prevented by IFN-γ. The suppressive effect was re-established with high doses of TGF-β1 in cultures, indicating that biological effects of TGF-β1 vary depending on nitric oxide (NO) production, its concentration in the microenvironment and regulation of the cytokine network. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 103
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15892256