SP1/miR-92a-1–5p/SOCS5: A novel regulatory axis in feline panleukopenia virus replication.

MicroRNAs (miRNAs) are vital post-transcriptional regulators that participate in host-pathogen interactions by modulating the expression of cellular factors. Previous studies have demonstrated that feline panleukopenia virus (FPV) alters miRNA expression levels within host cells. However, the relationship between FPV replication and host miRNAs remains unclear. Here, we demonstrated that FPV infection significantly altered cellular miR-92a-1–5p expression in F81 cells by upregulating the expression of specificity protein 1 (SP1). Furthermore, we observed that miR-92a-1–5p enhanced interferon (IFN-α/β) expression by targeting the suppressors of cytokine signaling 5 (SOCS5) that negatively regulates NF-κB signaling and inhibits FPV replication in host cells. These findings revealed that miR-92a-1–5p plays a crucial role in host defense against FPV infection. • FPV significantly altered miR-92a-1–5p expression in F81 cells by upregulating the expression of SP1. • SP1 is a vital transcriptional factor for miR-92a-1–5p expression. • MiR-92a-1–5p activated the NF-κB signaling pathway to promote IFN-I expression and inhibit FPV replication. [ABSTRACT FROM AUTHOR]