Back to Search
Start Over
Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra.
- Source :
-
Molecules . Sep2022, Vol. 27 Issue 17, p5552. 13p. - Publication Year :
- 2022
-
Abstract
- The COVID-19 pandemic continues to impose a huge threat on human health due to rapid viral mutations. Thus, it is imperative to develop more potent antivirals with both prophylactic and treatment functions. In this study, we screened for potential antiviral compounds from Sarcandra glabra (SG) against Cathepsin L and HIV-1 proteases. A FRET assay was applied to investigate the inhibitory effects and UPLC-HRMS was employed to identify and quantify the bioactive components. Furthermore, molecular docking was carried out to get a glimpse of the binding of active compounds to the proteases. Our results showed that the SG extracts (SGW, SG30, SG60, and SG85) inhibited HIV-1 protease with an IC50 of 0.003~0.07 mg/mL and Cathepsin L protease with an IC50 of 0.11~0.26 mg/mL. Fourteen compounds were identified along with eight quantified from the SG extracts. Chlorogenic acid, which presented in high content in the extracts (12.7~15.76 µg/mg), possessed the most potent inhibitory activity against HIV-1 protease (IC50 = 0.026 mg/mL) and Cathepsin L protease (inhibition: 40.8% at 0.01 mg/mL). Thus, SG extracts and the active ingredients could potentially be used to prevent/treat viral infections, including SARS-CoV-2, due to their dual-inhibition functions against viral proteases. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HIV
*VIRAL mutation
*VIRUS diseases
*CHLOROGENIC acid
*MOLECULAR docking
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 27
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Molecules
- Publication Type :
- Academic Journal
- Accession number :
- 159032689
- Full Text :
- https://doi.org/10.3390/molecules27175552