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Protective effects of chlorogenic acid on inflammatory responses induced by Staphylococcus aureus and milk protein synthesis in bovine mammary epithelial cells.

Authors :
Ji, Qiang
Zhang, Meng
Wang, Yanfeng
Chen, Yuhao
Wang, Liping
Lu, Xinyue
Bai, Linfeng
Wang, Manshulin
Bao, Lili
Hao, Huifang
Wang, Zhigang
Source :
Microbial Pathogenesis. Oct2022, Vol. 171, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

Staphylococcus aureus (S. aureus) is a major mastitis-causing pathogen in dairy cows. Dairy cows with mastitis suffer from a decrease in milk yield and protein content. Chlorogenic acid (CGA) is a natural product with anti-inflammatory effects. In this study, we examined the function and mechanism of CGA with regard to its anti-inflammatory effects and evaluated its protective function in milk protein synthesis in bovine mammary epithelial cells (BMECs). BMECs were cultured with and without infection by S. aureus and CGA, and extracellular inflammatory cytokines and amino acids in the medium and milk proteins were determined by ELISA. The function of IL- 10RA in anti-inflammatory processes and of SF-1 in milk protein synthesis was assessed by gene silencing. The activity of mTORC1, NF-κB, and STAT5 was examined by western blot. S. aureus caused intracellular infection and upregulated TNF-α, IL-1β, IL-6, and IL-8, whereas uptake of amino acids and milk protein synthesis were suppressed. CGA mitigated the S. aureus- induced inflammatory response and milk protein synthesis in vitro and in vivo. CGA alleviated S. aureus- induced inhibition of mTORC1 and STAT5 and upregulated IL-10 and IL- 10RA. In addition, SF-1 was predicted to be a transcription factor of the milk protein-encoding genes α-LA , β-LG , and CSN2. S. aureus downregulated SF-1 and CGA reversed the decline in milk protein synthesis due to SF-1 knockdown. Thus, CGA mitigates the inflammatory response that is induced by S. aureus and protects the uptake of amino acids and milk protein synthesis in BMECs. • S. aureus stimulates inflammatory response and decreases milk protein synthesis in bovine mammary epithelial cells. • Chlorogenic acid mitigates S. aureus -induced inflammatory response and the decline of milk protein synthesis. • CGA enhances mTORC1 signaling and the expression of IL-10 and IL-10RA [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08824010
Volume :
171
Database :
Academic Search Index
Journal :
Microbial Pathogenesis
Publication Type :
Academic Journal
Accession number :
159056433
Full Text :
https://doi.org/10.1016/j.micpath.2022.105726