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Enantioseparation and quantitative analysis of three alkaloids in Rhizoma Corydalis by chiral LC-MS/MS.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Oct2022, Vol. 220, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
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Abstract
- A chiral liquid chromatography-mass spectrometry (LC-MS/MS) approach was developed and verified for the first time in order to quantify the three alkaloids, namely tetrahydropalmatine (THP), tetrahydroberberine (THB) and tetrahydrocoptisine (THC) in Rhizoma Corydalis. Solid-phase extraction was used to prepare sample solution. Enantiomeric separation was obtained on a Chiralpak IC column using acetonitrile-water-ammonia (90:10: 0.1, v/v/v) as mobile phase. The detection was carried out in multiple reaction monitoring (MRM) mode with positive electrospray ionization source. Transitions of m/z 356.0→192.0, m/z 340.0→176.0 and m/z 324.1→176.0 were monitored for THP, THB and THC respectively. All calibration curves showed good linearity (r2 ≥0.9994) within the test ranges. The lower limit of quantification (LLOQ) was 1.0 ng mL−1 and 1.5 ng mL−1 for (+)- and (−)-THP, 1.0 ng mL−1 and 3.0 ng mL−1 for (+)- and (−)-THB, 1.5 ng mL−1and 1.8 ng mL−1 for (+)- and (−)-THC, respectively. The precision, repeatability, and stability tests all meet the requirements. The average recoveries of the analytes ranged from 98.9 % to 101.3 %. Ultimately, the established method was successfully applied to the quantitative analysis of these alkaloid enantiomers extracted from Rhizoma Corydalis of different habitats. [Display omitted] • Three alkaloids in Rhizoma Corydalis were separated with a new chiral method. • The lower limit of quantitation ranged from 1.0 ng mL−1 to 3.0 ng mL−1. • The method shows higher sensitivity compared with previous studies. • First report on quantitative analysis of chiral alkaloids from different habitats. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 220
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 159075698
- Full Text :
- https://doi.org/10.1016/j.jpba.2022.115008