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Arabidopsis thaliana E3 ligase AIRP4 is involved in GA synthesis.
- Source :
-
Journal of Plant Physiology . Oct2022, Vol. 277, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- Arabidopsis abscisic acid ABA-Insensitive RING Proteins (AtAIRP1–4) are RING E3s that play significant roles in ABA-signaling pathways. However, it is still unclear whether they have other functions. Here, AtAIRP4 was determined to play a role in response to gibberellin A3 (GA 3) in Arabidopsis thaliana. After proAtAIRP4::GUS transgenic lines were treated with GA 3 , the GUS activity decreased in hypocotyls. Increased hypocotyl elongation in response to GA 3 seen in WT was not observed in the AtAIRP4 -overexpression lines, whereas AtAIRP4 -overexpression lines were hypersensitive to Paclobutrazol (PAC, an inhibitor of GA biosynthesis) during the seed germination stage. Additionally, AtAIRP4 -overexpressing lines showed the lowest level of primary root elongation in the presence of GA 3. The levels of endogenous GA 3 in 35S::AtAIRP4 lines were lower than those in wild-type. In addition, among the plants, the mRNA levels of the GA synthetic gene GIBBERELLIN 20-OXIDASE1 (GA20ox1) was the lowest in overexpressing line. However, the expression of the response gene DELLA RGA-LIKE3 (RGL3) was the highest in overexpressing lines after treatment with GA 3. Thus, AtAIRP4 plays a negative role in GA-mediated hypocotyl elongation and root growth, and it inhibits the synthesis of endogenous biologically active GA 3 to some extent. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01761617
- Volume :
- 277
- Database :
- Academic Search Index
- Journal :
- Journal of Plant Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 159140475
- Full Text :
- https://doi.org/10.1016/j.jplph.2022.153805