CRISPR/Cas9‐mediated generation of fls2 mutant in Nicotiana benthamiana for investigating the flagellin recognition spectrum of diverse FLS2 receptors.

In addition, we combined transient expression and ROS burst assays to rapidly validate the FLS2 flagellin epitope recognition spectrum from 29 plant species in an I N. benthamiana FLS2 i double-mutant. CRISPR/Cas9-mediated generation of fls2 mutant in Nicotiana benthamiana for investigating the flagellin recognition spectrum of diverse FLS2 receptors Keywords: Flagellin-sensing 2; flagellin epitope; Nicotiana benthamiana; CRISPR/Cas9; transient assay; reactive oxygen species burst EN Flagellin-sensing 2 flagellin epitope Nicotiana benthamiana CRISPR/Cas9 transient assay reactive oxygen species burst 1853 1855 3 09/23/22 20221001 NES 221001 Plant cell surface pattern-recognition receptors (PRRs) mount pattern-triggered immunity (PTI) by recognizing the typical molecular structures of pathogens, termed pathogen-associated molecular patterns (PAMPs), providing the first line of defence against various phytopathogens. [Extracted from the article]