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Self-assembly strategy to reduce non-specific adsorption for the development of high sensitivity quantitative immunoassay.
- Source :
-
Analytica Chimica Acta . Oct2022, Vol. 1229, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- The development of functionalized surfaces with low non-specific adsorption is important for their biomedical applications. To inhibit non-specific adsorption on glass substrate, we designed a novel optical biochip by modifying a layer of dense negatively charged film (SO 3 2−) on its substrate surface via self-assembly. Compared with the untreated glass substrate, it reduced the adsorption by about 300-fold or 400-fold by poly (styrene sulfonic acid) sodium salt (PSS), or meso -tetra (4-sulfonatophenyl) porphine dihydrochloride (TSPP) on individually the modified glass substrate. Considering the effect of fluorescence resonance energy transfer (FRET) between TSPP and the QDs in solution by mixing, a strategy of 2-layer of TSPP followed by 4-layer of PSS was designed to modify the glass for preparing biochips. Under the optimized conditions, the biochip on functionalized glass substrate co-treated with TSPP and PSS realized the sensitive quantitative detection of C-reactive protein (CRP) based on a quantum dot fluorescence immunosorbent assay (QD-FLISA). The limit of detection (LOD) for CRP achieved 0.69 ng/mL with the range of 1-1,000 ng/mL using TSPP and PSS co-treated glass substrate surface, which was respectively about 1.9-fold and 7.5-fold more sensitive to the PSS-modified biochip and the TSPP-modified biochip. This work demonstrated an effective and convenient strategy to obtain biochips with low non-specific adsorption properties on functionalized surfaces, thus providing a new approach for creating ultra-high sensitivity microchannels or microarrays on glass substrates. [Display omitted] • The causes of non-specific adsorption on glass substrates were studied. • The dense negatively charged polymer substrates (SO 3 2−) were prepared and evaluated. • A novel optical biochip co-treated with TSPP and PSS was constructed to inhibit non-specific adsorption. • The designed biochip can realize the sensitive quantitative detection of CRP based on QD-FLISA. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENCE resonance energy transfer
*ADSORPTION (Chemistry)
*IMMUNOASSAY
Subjects
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 1229
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 159268489
- Full Text :
- https://doi.org/10.1016/j.aca.2022.340367