基因表达谱芯片分析续答健骨方治疗去卵巢大鼠骨质 疏松症的基因表达差异.

Objective To screen the differentially expressed genes of Xuling Jiangu Prescription in osteoporosis model rats by gene expression profile chip. Methods 15 rats were divided into three groups according to random number method : sham operation group, model group and Xuling Jiangu Fang group, with 5 rats in each group.The experimental group was given Xuling Jiangu Prescription according to body weight, and the other two groups were given the same volume of normal saline by intragastric administration, once a day, for 13 consecutive weeks. After 13 weeks, the femur of the left leg of the rat was taken for gene expression profile microarray detection, and the differentially expressed genes between groups were obtained by microarray analysis, and their functions and pathways were analyzed. Results Ten genes with significantly decreased expression after modeling but significantly increased expression after medication were screened, and 218 genes with significantly increased expression after modeling but significantly decreased expression after medication were screened under the conditions of | log2 Fold change | ≥ 1, Q < 0. 05). Enrichment analysis of KEGG signaling pathway revealed that PI3K/Akt pathway was one of the key pathways. The differential genes RplpO and Cnpy2 were further screened under the following conditions; | log2 Fold change | ≥ 2, q < 0. 01). Conclusion The therapeutic mechanism of Xuling Jiangu Prescription may be related to PI3K/AKT signaling pathway, RplpO and Cnpy2 genes. [ABSTRACT FROM AUTHOR]