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IDENTIFICATION OF DEREPRESSED AUTOIMMUNOCOMPETENT B LYMPHOID CELLS IN NZB MICE.
- Source :
-
Clinical & Experimental Immunology . Mar1974, Vol. 16 Issue 3, p431-443. 13p. - Publication Year :
- 1974
-
Abstract
- Plaque assays for derepressed autoimmunocompetent B lymphoid cells with specificities for two murine erythrocyte surface autoantigens have been developed. Using purified anti-HB and anti-X anti-erytlirocyte autoantibodies, fluid-phase complement fixation reactions were performed to determine the influence of pH and ionic strength on the efficiency of autoantibody-mediated cytolysis of murine erythrocytes. Anti-HB autoantibody exhibited optimal cytolysis of bromelin-treated erythrocytes at pH 7.0, ionic strength 0.15; haemolysis of intact erythrocytes by anti-X autoantibody was observed over a broad range with maximum sensitivity and specificity at pH 6.6, ionic strength 0.14. NZB spleen ceils secreting anti-HB autoantibody were detected in modified direct haemolysis-in-gel assays, were neutralized by soluble HB autoantigen, and appeared to represent derepressed B lymphoid cells of anti-HB type. Anti-X plaque-forming cells, not demonstrable using conventional assays, were detected under conditions corresponding to the pH and ionic strength optima determined for complement-dependent cytolysis using purified autoantibody. NZB spleen cells secreting anti-X autoantibody were detected in indirect assays employing monolayers of erythrocytes, were neutralized by soluble X autoantigen, and appeared to represent derepressed B lymphoid cells of anti-X type. The distribution of observed plaque diameters in both HB and X assays suggested that more than 95% of all cells secreting autoantibody were detected. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 16
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15947837