CDDO-Me'nin Meme Kanseri Hücrelerindeki Etkilerinin Tamoxifen ve Docetaxel ile Karşılaştırılması.

Objectives: Synthetic triterpenoids derived from oleanolic acid are known to have strong antiproliferative and antitumorigenic activity. The aim of this study is to investigate the the cytotoxic effects of CDDO-Me, a triterpenoid, on human breast cancer cell lines by comparing it with Tamoxifen and Docetaxel, which are used in the routine treatment of breast cancer. Material- Method: MCF-7 and MDA MB-231 were preferred as breast cancer cells to study the cytotoxic impressions of CDDO-Me, Docetaxel and Tamoxife. By applying various doses of each drug to both cells, its cytotoxic effects were determined by xCELLigence. Cell blocks were equipped and scoring the expressions of Ki-67 and Cyclin-D1 (proliferation index), antiapoptotic Bcl-2, proapoptotic Bax by histological and immunohistochemical staining in the blocks. Results: CDDO-Me, Docetaxel and Tamoxifen inhibited the cell viability of both cells. When the effects of CDDO-Me with Tamoxifen and Docetaxel were compared at the molecular level, it was concluded that CDDO-Me was statistically more effective than Tamoxifen and Docetaxel in terms of apoptosis pathway in both cell lines (p<0.001). In terms of cell cycle, CDDO-Me showed statistically similar effects with Tamoxifen in MCF-7 cells and Docetaxel was statistically more effective in this cell line (p<0.001) and CDDO-Me showed statistically similar effects with Docetaxel in MDA MB-231 cells and Tamoxifen was statistically more effective in this cell line (p<0.001). Conclusion: Although the antiproliferative and apoptosis-inducing effects of CDDO-Me differ in both types of breast cancer according to the effects of Tamoxifen and Docetaxel used in routine breast cancer treatment, CDDO-Me may be an alternative chemotherapeutic agent in the treatment of breast cancer and results of this study may guide in vivo studies in future. [ABSTRACT FROM AUTHOR]