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Fluorescence enhancement of near infrared cell membrane probe by β-cyclodextrin supramolecular interaction.
- Source :
-
Dyes & Pigments . Nov2022, Vol. 207, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- In this paper, oxazolepyridine and coumarin units were combined to prepare cell membrane fluorescent probe (PM-A) through aldol condensation reaction. PM-A had long wavelength emission (665–739 nm), high molar extinction coefficient (around 3.14 × 104 M−1cm−1) and large Stokes shift (150–219 nm). The photo-stability test showed that PM-A had a high relative residual absorption (93%) after 6 h of continuous illumination. In addition, the supramolecular interaction between PM-A and β-cyclodextrin was evaluated and applied to cell imaging. Probe PM-A had no fluorescence in water, but had obvious fluorescence signal in β-cyclodextrin solution. With the addition of β-cyclodextrin (0–800 eq), the fluorescence quantum yield of PM-A increased from 0% to 45%. Laser confocal experiments showed that when only PM-A was added, the probe concentration needed to be 15 μM to image the cell membrane obviously. In sharp contrast, with the help of β-cyclodextrin solution, the concentration of probe PM-A only needed 0.5 μM to quick and high specificity image cell membranes. Moreover, PM-A had high photo-bleaching resistance and long retention time after adding β-cyclodextrin. These excellent properties of PM-A were of great significance to the study of cell membrane. [Display omitted] • A near-infrared fluorescent probe (PM-A) with oxazolepyridine and coumarin units was prepared for the imaging of cell membrane at 15 μM. • The supramolecular interaction between probe PM-A and β-cyclodextrin was evaluated. • Probe PM-A showed rapid and high specificity of cell membrane imaging at the concentration of 0.5 μM with the help of β-cyclodextrin. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CYCLODEXTRINS
*CELL membranes
*FLUORESCENCE yield
*CELL imaging
*FLUORESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 01437208
- Volume :
- 207
- Database :
- Academic Search Index
- Journal :
- Dyes & Pigments
- Publication Type :
- Academic Journal
- Accession number :
- 159569110
- Full Text :
- https://doi.org/10.1016/j.dyepig.2022.110693