Back to Search Start Over

Growth Kinetics Monitoring of Gram-Negative Pathogenic Microbes Using Raman Spectroscopy.

Authors :
Saikia, Dimple
Jadhav, Priyanka
Hole, Arti R.
Krishna, Chilakapati Murali
Singh, Surya P.
Source :
Applied Spectroscopy. Oct2022, Vol. 76 Issue 10, p1263-1271. 9p.
Publication Year :
2022

Abstract

Optical density based measurements are routinely performed to monitor the growth of microbes. These measurements solely depend upon the number of cells and do not provide any information about the changes in the biochemical milieu or biological status. An objective information about these parameters is essential for evaluation of novel therapies and for maximizing the metabolite production. In the present study, we have applied Raman spectroscopy to monitor growth kinetics of three different pathogenic Gram-negative microbes Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii. Spectral measurements were performed under 532 nm excitation with 5 seconds of exposure time. Spectral features suggest temporal changes in the "peptide" and "nucleic acid" content of cells under different growth stages. Using principal component analysis (PCA), successful discrimination between growth phases was also achieved. Overall, the findings are supportive of the prospective adoption of Raman based approaches for monitoring microbial growth. Graphical Abstract [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00037028
Volume :
76
Issue :
10
Database :
Academic Search Index
Journal :
Applied Spectroscopy
Publication Type :
Academic Journal
Accession number :
159579367
Full Text :
https://doi.org/10.1177/00037028221109624