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Bioprocess for Hyperactive Thermotolerant Aspergillus fumigatus Phytase and its Application in Dephytinization of Wheat Flour.
- Source :
-
Catalysis Letters . Nov2022, Vol. 152 Issue 11, p3220-3232. 13p. - Publication Year :
- 2022
-
Abstract
- In the present study, statistically optimized production of thermotolerant Aspergillus fumigatus phytase was carried out in batch fermentation (1-L). An enhancement in phytase production was noted up to 3.3-folds as compared to unoptimized and using One Variable at a Time strategy. Phytase was purified up to 26-folds with a single band of ~ 62 kDa. The optimum activity of purified phytase was recorded at pH 5.5 and 65 °C. The kinetic parameters, Km and Vmax, were observed to be, 0.75 mM, and 345 µmol/min/mg-protein,respectively. The activity was constrained by silver and mercuric ions and slightly increased by calcium ions. The phytase showed high dephytinization up to 78.7% of wheat flour (10 g) at a small reaction volume (30 mL). On up-scaling to 3-L reaction, a slight decrease in dephytinization to 72.5% was recorded. This is the first report where hyperactive purified phytase from thermotolerant A. fumigatus has been demonstrated to degrade wheat flour-based phytic acid up to a 3-L scale at the bioreactor level. This finding can be explored at a commercial scale for multi-dimensional future biotechnological applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1011372X
- Volume :
- 152
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Catalysis Letters
- Publication Type :
- Academic Journal
- Accession number :
- 159684687
- Full Text :
- https://doi.org/10.1007/s10562-021-03886-0