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Dissection of the response mechanism of alfalfa under phosphite stress based on metabolomic and transcriptomic data.

Authors :
Li, Zhenyi
Wu, Yao
Hu, Jingyun
Yang, Guofeng
Wang, Zengyu
Sun, Juan
Source :
Plant Physiology & Biochemistry. Dec2022, Vol. 192, p35-49. 15p.
Publication Year :
2022

Abstract

Phosphite, a reduced form of phosphate, inhibits the growth and even has toxic effect on plants. To learn more about the mechanism of alfalfa responses to phosphite, the morphological and physiological characteristics, and the metabolites and transcript levels were comprehensively analyzed following the exposure of alfalfa seedlings to phosphite and phosphate under greenhouse conditions. The results showed that phosphite inhibited seedling growth and photosynthesis. However, the absorption efficiency of phosphite was higher than that of phosphate in roots, which was supported by increased total phosphorus concentration of 16.29% and 52.30% on days 8 and 12. Moreover, phosphite stress affected the synthesis of lipids and carbohydrates, which were reflected in enhanced glycolipid and sulfolipid in roots and amylose in shoots. Phosphite stress resulted in a decrease in indole acetic acid (IAA) in the whole plant and zeatin in the shoots, which could enable alfalfa to adapt to the phosphite environment. Some genes involved in phosphate starvation response included SPX , phosphate response regulator2 , and inorganic phosphate transporter 1–4 (PHT1;4) in roots were affected by phosphite stress. In addition, some genes that are involved in stress responses and DNA repair were induced by phosphite stress. These observations together suggest that alfalfa responds to phosphite stress by inhibiting growth, regulating the genes induced by phosphate starvation, improving oxidative protection, promoting DNA repair, and adjusting the IAA and zeatin signaling transductions. Our findings provide novel insights into the molecular response to phosphite stress in alfalfa. • Phosphite inhibited the growth of alfalfa seedlings. • Phosphite inhibited nitrogen assimilation and promoted sulfate assimilation. • Phosphite stress increased Phosphate starvation induced (PSI) genes. • Phosphite induced stress response genes and promoted DNA repair in alfalfa. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09819428
Volume :
192
Database :
Academic Search Index
Journal :
Plant Physiology & Biochemistry
Publication Type :
Academic Journal
Accession number :
159929364
Full Text :
https://doi.org/10.1016/j.plaphy.2022.09.024