刺五加多糖纳米乳与鸡法氏囊 苗配合对雏鸡免疫增强作用的研究.

The experiment was to study the growth performance and immune-enhancing effects of Acanthopanax senticosus polysaccharide nanoemulsion combined with chicken bursal inactivated vaccine on chicks, to screen the optimal immune-enhancing dose of Acanthopanax senticosus polysaccharide nanoemulsion as adjuvant on chicken bursal inactivated vaccine. A total of 140 one-day-old AA broilers were randomly divided into seven groups, with four replicates in each group and five broilers in each replicate. The broilers in ASPS-NE (L) group were injected with 0.2 mL of Acanthopanax senticosus polysaccharide nanoemulsion (32 g/L), the broilers in ASPS-NE (M) group were injected with 0.4 mL of Acanthopanax senticosus polysaccharide nanoemulsion (64 g/L), the broilers in ASPS-NE (H) group were injected with 0.6 mL of Acanthopanax senticosus polysaccharide nanoemulsion (128 g/L), the bursal vaccine alone group was injected with 0.3 mL bursal vaccine, the blank group was injected with 0.3 mL normal saline, the positive control group was injected with 10 g/L Astragalus extract 0.4 mL, and the chickens in blank nanomilk group were injected with 0.4 mL nanomilk. All groups were immunized for the first time on 14 d, followed by two immunizations and three immunizations on 21 and 28 d. The experiment lasted for 35 d. The results showed that compared with pure vaccine group, blank control group and blank nano group, the average daily gain of chicks in ASPS-NE (M) group and positive group was extremely increased (P<0.01), and feed to gain ratio was extremely decreased (P<0.01). Acanthopanax senticosus polysaccharide nanoemulsion as adjuvant combined with chicken bursal inactivated vaccine could promote the production of antibodies to chicken bursal inactivated vaccine, increase the immune organ index and the expression level of IL-2, IL-4 and IFN-γ of chicks. Medium dose of Acanthopanax senticosus polysaccharide nanoemulsion had the best effect. The experiment indicates that the nano-emulsion of Acanthopanax senticosus polysaccharide has a good immune-promoting effect as an immune adjuvant of chicken bursa of Bursa inactivated vaccine, and the concentration of 64 g/L has a good immuneenhancing effect. [ABSTRACT FROM AUTHOR]