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Salvianolic Acid Ameliorates Pressure Overload-Induced Cardiac Endothelial Dysfunction via Activating HIF1α/HSF1/CD31 Pathway.

Authors :
Li, Na
Hang, Weijian
Shu, Hongyang
Wen, Zheng
Ceesay, Bala Musa
Zhou, Ning
Source :
American Journal of Chinese Medicine. 2022, Vol. 50 Issue 7, p1869-1885. 17p.
Publication Year :
2022

Abstract

Pressure overload is a major risk factor for various cardiovascular diseases. Disorders of the endothelium are involved in the pathological mechanisms of pressure, and maintaining endothelial function is a practical strategy to alleviate pressure overload-induced cardiac injury. In this study, we provided evidence that salvianolic acid, the active component of Danshen, a traditional Chinese herb medicine, preserved pressure overload-induced cardiac dysfunction via protecting endothelium. Male C57BL/6J mice were imposed with transverse aortic constriction to mimic pressure overload and treated with salvianolic acid (200 mg/kg/day) or vehicle for 6 weeks. The hemodynamic and cardiac functional parameters were detected by the cardiac catheter and transthoracic echocardiography. The pathological measurements were conducted by heart hematoxylin-eosin, wheat germ agglutinin staining, Masson's trichrome staining, and immunofluorescence staining. Endothelial cell (EC) proliferation was estimated using the Cell Counting Kit-8, EC migration was evaluated by scratched assay, and EC integrity was observed by electron microscope. Salvianolic acid notably inhibited cardiac chamber enlargement, restrained cardiac contractile dysfunction, and repressed cardiac fibrosis caused by chronic pressure overload. Salvianolic acid maintained endothelial tight junction integrity by boosting the expression of CD31. Furthermore, the endothelial protective effect of salvianolic acid against pressure overload is dependent on the activation of hypoxia-inducible factor 1 α , which consequently activated heat shock factor 1 and promoted CD31 expression. Our study uncovered that salvianolic acid protected cardiac ECs against pressure overload via a HIF1 α /HSF1/CD31 pathway, indicating a potential appliance of salvianolic acid in hypertensive heart disease. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0192415X
Volume :
50
Issue :
7
Database :
Academic Search Index
Journal :
American Journal of Chinese Medicine
Publication Type :
Academic Journal
Accession number :
160176082
Full Text :
https://doi.org/10.1142/S0192415X22500793