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Obesity promotes lipid accumulation in lymph node metastasis of gastric cancer: a retrospective case‒control study.
- Source :
-
Lipids in Health & Disease . 11/17/2022, Vol. 21 Issue 1, p1-14. 14p. - Publication Year :
- 2022
-
Abstract
- Background: The connection between obesity, lipid accumulation, and lymph node metastasis (LNM) in gastric cancer (GC) is unclear. Methods: The association of body mass index (BMI) and serum lipid levels with LNM was measured by calculating the odds ratio (OR) and 95% confidence interval (CI) in 1,058 eligible GC patients with a mean age of 61.4 years. Meanwhile, differentially expressed genes (DEGs) were identified between lymph node metastasis-positive (N +) and -negative (N0) groups using public RNA-seq data. Neutral lipids in human GC samples were detected by Oil red O staining. The expression of cluster of differentiation 36 (CD36), fatty acid synthase (FASN), and lipoprotein lipase (LPL) was detected by immunohistochemistry (IHC) and quantitative real-time PCR. Results: Compared with normal-weight patients, overweight (OR = 2.02, 95% CI = 1.26–3.23) and obese (OR = 1.83, 95% CI = 1.15–2.91) patients showed increased ORs for LNM. However, no significant results were obtained for serum lipids in the multivariable-adjusted model (P > 0.05). Subgroup analysis suggested that increased low-density lipoprotein cholesterol was a risk factor in females (OR = 1.27, 95% CI = 1.02–1.59). Functional enrichment analysis of DEGs revealed a connection between lipid metabolism and LNM. Meanwhile, lipid staining showed a mass of lipids in obese N + tumor samples, and IHC analysis indicated an increase in LPL and CD36 expression in N + cases, implying a crucial role for exogenous lipid supply in LNM. Conclusions: High BMI significantly increases the risk of LNM in GC and promotes lipid accumulation in GC cells in LNM. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1476511X
- Volume :
- 21
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Lipids in Health & Disease
- Publication Type :
- Academic Journal
- Accession number :
- 160293496
- Full Text :
- https://doi.org/10.1186/s12944-022-01734-7