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Development of a colloidal gold immunochromatographic strip for rapid and sensitive detection of nicotine.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Jan2023, Vol. 223, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Nicotine (NCT) is commonly contained in e-cigarette oils, and long-term nicotine intake can exacerbate cardiovascular disease, neurodegenerative disease, and lung cancer. Therefore, there is a need to develop a fast and sensitive method for detecting NCT in e-cigarette oils. We prepared anti-NCT monoclonal antibodies (mAbs), which belong to the IgG2 subclass. The optimum mAb had a half-maximal inhibitory concentration (IC 50) of 6.81 ng/mL for NCT. We developed a colloidal gold immunochromatographic strip (CG-ICS) to detect NCT content with a visual cut-off and limit of detection of 20 ng/mL and 0.40 ng/mL, respectively. The recovery rates of NCT from spiked samples ranged between 100.36% and 109.96% based on CG-ICS. These results were consistent with those obtained from high performance liquid chromatography (HPLC). We confirmed the reliability of CG-ICS using NCT-positive samples. Therefore, CG-ICS represents an effective and rapid on-site screening tool for the detection of NCT in e-cigarette oils. • Anti-NCT mAbs were prepared, which had a IC 50 of 6.81 ng/mL for NCT. • CG-ICS was developed to detect NCT content with a visual cut-off and LOD of 20 ng/mL and 0.40 ng/mL, respectively. • The recovery rates of NCT from spiked samples ranged between 100.36% and 109.96% based on CG-ICS. • These results were consistent with those obtained from HPLC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 223
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 160365489
- Full Text :
- https://doi.org/10.1016/j.jpba.2022.115132