A dual-site multifunctional fluorescent probe for selective detection of endogenous H2O2 and SO2 derivatives based on ICT process and its bioimaging application.

A dual-site multifunctional fluorescent probe for selective detection of endogenous H 2 O 2 and SO 2 derivatives based on ICT process and its bioimaging application. [Display omitted] • A dual-site multifunctional probe was designed for detection of endogenous H 2 O 2 and SO 3 2−. • The sensing mechanism was confirmed by 1H NMR and theoretical calculation data. • The probe was successfully used for bioimaging of endogenous H 2 O 2 and SO 3 2−. In this paper, we reported a coumarin-based fluorescent probe for selective detection of H 2 O 2 /SO 2 derivatives via ICT process. To the best of our knowledge, it was few reported with the same probe to enable visual detection of H 2 O 2 /SO 2 derivatives in vivo and in vitro. H 2 O 2 and SO 3 2− were selectively sensed over other analytes, and the probe displayed 20-fold and 220-fold relative fluorescence intensity respectively, as well as the good linear relationship and the excellent detection limits of 2.7 * 103 nM and 19.3 nM. Furthermore, the probe was successfully used for fluorescence imaging of the HeLa cells and the mice to monitor exogenous and endogenous H 2 O 2 and SO 3 2−, suggesting its potential biomedical application for investigation and detection the intermediate indicator of oxidative stress in vitro and in vivo. [ABSTRACT FROM AUTHOR]