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EXTRACTION, PURIFICATION AND CHARACTERIZATION OF LIPASE FROM THE DIGESTIVE DUCT OF COMMON CARP CYPRINUS CARPIO L.
- Source :
-
Iraqi Journal of Agricultural Sciences . 2022, Vol. 53 Issue 5, p1011-1020. 10p. - Publication Year :
- 2022
-
Abstract
- This study was aimed to use digestive duct of common carp Cyprinus Carpio L. as economic source for fish lipase extraction . The enzyme was extracted using potassium phosphate buffer (pH 7, 0.2 M ). The crude extract then precipitated at the saturation range between 40 – 80 % and dialyzed . Further purified carried out through ion exchange chromatography using DEAE-Cellulose column (2.5 × 30 cm) and gel filtration chromatography by Sephadex G-100 column (1.5 x 60 cm). The purified lipase had a molecular weight of 37.150KDa,and optimum pH and temperature for enzyme activity about 45◦C and 6.5 using olive oil as substrate .The specific activity, number of purification folds and yield of purified Lipase were 146.48U/mg, 9.76 and 40.50% respectively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00750530
- Volume :
- 53
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Iraqi Journal of Agricultural Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 160409276
- Full Text :
- https://doi.org/10.36103/ijas.v53i5.1615