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MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone.
- Source :
-
Growth Factors . Nov2022, Vol. 40 Issue 5/6, p175-185. 11p. - Publication Year :
- 2022
-
Abstract
- This study aimed to explore the specific effect of miR-200c in anaplastic thyroid cancer (ATC). Hth74 and ARO cell lines were used. Proliferation, invasion, and colony formation activities of Hth74 and ARO cell lines affected by miR-200c were studied. Expression of epithelial-to-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, Slug, and Snail) in the Hth74 and ARO cell lines were validated by western blot and qRT-PCR. In addition, the regulation of the parathyroid hormone-like hormone (PTHLH) by miR-200c was assessed. Overexpression of miR-200c inhibited the invasion, proliferation, and colony formation of the ATC cell lines, whereas its downregulation achieved the opposite results. PTHLH was found to be regulated negatively by miR-200c through a miR-200c binding site within the 3′-UTR of PTHLH. miR-200c repressed the proliferation, invasion, and EMT process of cells in ATC cell lines by targeting PTHLH post-transcriptionally, which indicates that miR-200c may be a potential target for the treatment of ATC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08977194
- Volume :
- 40
- Issue :
- 5/6
- Database :
- Academic Search Index
- Journal :
- Growth Factors
- Publication Type :
- Academic Journal
- Accession number :
- 160454537
- Full Text :
- https://doi.org/10.1080/08977194.2022.2108809